The Wood–Ljungdahl pathway is a set of biochemical reactions used by some bacteria and archaea calledacetogens. It is also known as the reductive acetyl-coenzyme A (Acetyl-CoA) pathway.[1] This pathway enables these organisms to use hydrogen as an electron donor, and carbon dioxide as an electron acceptor and as a building block for biosynthesis.
In this pathway carbon dioxide is reduced to carbon monoxide and formic acid or directly into a formyl group, the formyl group is reduced to a methyl group and then combined with the carbon monoxide and Coenzyme A to produce acetyl-CoA. Two specific enzymes participate on the carbon monoxide side of the pathway: CO Dehydrogenase andacetyl-CoA synthase. The former catalyzes the reduction of the CO2 and the latter combines the resulting CO with a methyl group to give acetyl-CoA.[1][2]
The pathway occurs in bacteria and archaea, e.g. methanogens[3] and in acetate-producing bacteria such asClostridium. Unlike the Reverse Krebs cycle and the Calvin cycle, this process is not cyclic. A recent study of the genomes of a set of bacteria and archaea suggests that the last universal common ancestor (LUCA) of all cells was using the Wood–Ljungdahl pathway in a hydrothermal setting.[4]